Li, Bo and Lu, Min and Jin, Lei and Zheng, Maoen and Sun, Peilu and Lei, Shanshan and Xiong, Shan and Chen, Suhong (2019) Simultaneous Quantification of Brigatinib and Brigatinib-Analog in Rat Plasma and Brain Homogenate by LC-MS/MS: Application to Comparative Pharmacokinetic and Brain Distribution Studies. International Journal of Analytical Chemistry, 2019. pp. 1-10. ISSN 1687-8760
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Abstract
Brigatinib and brigatinib-analog are potent and selective ALK inhibitors with the similar structure. A simple and sensitive high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of brigatinib and brigatinib-analog in rat plasma and brain homogenate was developed and validated. Chromatographic separation was carried out on an ODS column with acetonitrile and 0.1% formic acid in water as the mobile phase with gradient elution at a flow rate of 0.5 mL/min. Detections were performed using a TSQ Quantum Ultra mass spectrometric detector with electrospray ionization (ESI) interface, which was operated in the positive ion mode. A simple protein precipitation preparation process was used. The lower limits of quantification (LLOQs) were 1.0 ng/mL and 0.5 ng/mL for analytes in rat plasma and brain homogenate, respectively. The intrabatch and interbatch precision and accuracy of brigatinib and brigatinib-analog were well within the acceptable limits of variation. The simple and sensitive LC-MS/MS method was successfully applied to the pharmacokinetic and brain distribution studies following a single oral administration of brigatinib and brigatinib-analog to rats. The above studies would lay a good foundation for the further applications of brigatinib and brigatinib-analog.
Item Type: | Article |
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Subjects: | Pacific Library > Chemical Science |
Depositing User: | Unnamed user with email support@pacificlibrary.org |
Date Deposited: | 24 Jan 2023 06:22 |
Last Modified: | 15 Jun 2024 11:38 |
URI: | http://editor.classicopenlibrary.com/id/eprint/49 |