Li, Rui and Xia, Xianyou and Wang, Xing and Sun, Xiaoyu and Dai, Zhongye and Huo, Dawei and Zheng, Huimin and Xiong, Haiqing and He, Aibin and Wu, Xudong and Koo, Bon-Kyoung (2020) Generation and validation of versatile inducible CRISPRi embryonic stem cell and mouse model. PLOS Biology, 18 (11). e3000749. ISSN 1545-7885
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Abstract
Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated (Cas) 9 has been widely used far beyond genome editing. Fusions of deactivated Cas9 (dCas9) to transcription effectors enable interrogation of the epigenome and controlling of gene expression. However, the large transgene size of dCas9-fusion hinders its applications especially in somatic tissues. Here, we develop a robust CRISPR interference (CRISPRi) system by transgenic expression of doxycycline (Dox) inducible dCas9-KRAB in mouse embryonic stem cells (iKRAB ESC). After introduction of specific single-guide RNAs (sgRNAs), the induced dCas9-KRAB efficiently maintains gene inactivation, although it modestly down-regulates the expression of active genes. The proper timing of Dox addition during cell differentiation or reprogramming allows us to study or screen spatiotemporally activated promoters or enhancers and thereby the gene functions. Furthermore, taking the ESC for blastocyst injection, we generate an iKRAB knock-in (KI) mouse model that enables the shutdown of gene expression and loss-of-function (LOF) studies ex vivo and in vivo by a simple transduction of gRNAs. Thus, our inducible CRISPRi ESC line and KI mouse provide versatile and convenient platforms for functional interrogation and high-throughput screens of specific genes and potential regulatory elements in the setting of development or diseases.
Item Type: | Article |
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Subjects: | Pacific Library > Biological Science |
Depositing User: | Unnamed user with email support@pacificlibrary.org |
Date Deposited: | 12 Jan 2023 08:43 |
Last Modified: | 05 Jun 2024 10:37 |
URI: | http://editor.classicopenlibrary.com/id/eprint/39 |