New Therapy for Spinal Cord Injury: Autologous Genetically-Enriched Leucoconcentrate Integrated with Epidural Electrical Stimulation

Islamov, Rustem and Bashirov, Farid and Izmailov, Andrei and Fadeev, Filip and Markosyan, Vage and Sokolov, Mikhail and Shmarov, Maksim and Logunov, Denis and Naroditsky, Boris and Lavrov, Igor (2022) New Therapy for Spinal Cord Injury: Autologous Genetically-Enriched Leucoconcentrate Integrated with Epidural Electrical Stimulation. Cells, 11 (1). p. 144. ISSN 2073-4409

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Abstract

The contemporary strategy for spinal cord injury (SCI) therapy aims to combine multiple approaches to control pathogenic mechanisms of neurodegeneration and stimulate neuroregeneration. In this study, a novel regenerative approach using an autologous leucoconcentrate enriched with transgenes encoding vascular endothelial growth factor (VEGF), glial cell line-derived neurotrophic factor (GDNF), and neural cell adhesion molecule (NCAM) combined with supra- and sub-lesional epidural electrical stimulation (EES) was tested on mini-pigs similar in morpho-physiological scale to humans. The complex analysis of the spinal cord recovery after a moderate contusion injury in treated mini-pigs compared to control animals revealed: better performance in behavioural and joint kinematics, restoration of electromyography characteristics, and improvement in selected immunohistology features related to cell survivability, synaptic protein expression, and glial reorganization above and below the injury. These results for the first time demonstrate the positive effect of intravenous infusion of autologous genetically-enriched leucoconcentrate producing recombinant molecules stimulating neuroregeneration combined with neuromodulation by translesional multisite EES on the restoration of the post-traumatic spinal cord in mini-pigs and suggest the high translational potential of this novel regenerative therapy for SCI patients.

Item Type: Article
Subjects: Pacific Library > Biological Science
Depositing User: Unnamed user with email support@pacificlibrary.org
Date Deposited: 21 Jan 2023 06:00
Last Modified: 28 May 2024 06:10
URI: http://editor.classicopenlibrary.com/id/eprint/24

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